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文献和实验and enzymes that harbors the ability torecognize and cleave foreign nucleic acids based on their sequencesignature. Three known CRISPR systems, types I, II, and III, havebeen described so far [ 12 – 15 ]. Among these different CRISPRsystems, type II CRISPR
【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离
6). 5. S-gal LB powder (Sigma, St. Louis, MO) (see Note 7). 6. Solution I: 50 mM glucose, 25 mM Tris–HCl (pH 8.0), and10 mM EDTA (pH 8.0). Filter sterilized. Store at 4°C. 7. Solution II: 0.1 M NaOH and 1% SDS. Store at 4°C. 8. Solution
Combined Flow Cytometric Measurement of Two Cell-Surface Antigens and DNA-RNA Content
, without adverse effects. iii. Prepare a sample of cells stained only with PY (without any cell-surface or 7-AAD staining). This sample is necessary to determine the amount of fluorescence overlap of PY into other detectors
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