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文献和实验Inverse PCR & Cycle Sequencing
solution (P/N 50611), and the Perkin Elmer ABI BigDye Terminator Cycle Sequencing Ready Reaction Kit (P/N 4303154). The use of other thermal cyclers and sequencing strategies will require different cycling parameters and sequencing protocols.
Inverse PCR Cycle Sequencing of P Element Insertions for STS Generation
off the 3' end of the P-element. The latter primer combinations are therefore used in all initial experiments; the Pry2/Pry1 combination can be used in those cases where strong and unique bands do not result. Cycle on MJ Research Thermal Cycler using
Human Complement Components C4A and C4B Genetic Diversities: Complex Genotypes and Phenotypes
?primer single?cycle DNA polymerization (LSP); module?specific PCR GO TO THE FULL PROTOCOL: PDF or HTML at Wiley Online Library
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