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文献和实验SuperScript III One-Step RT-PCR System with Platinum® Taq High Fidelity
for 25 or 100 amplification reactions of 50 µl each. Note: This kit has been optimized for end-point RT-PCR. For quantitative real-time RT-PCR, use the SuperScript™ III Platinum® One-Step Quantitative RT-PCR System. 实验
Inverse PCR & Cycle Sequencing
solution (P/N 50611), and the Perkin Elmer ABI BigDye Terminator Cycle Sequencing Ready Reaction Kit (P/N 4303154). The use of other thermal cyclers and sequencing strategies will require different cycling parameters and sequencing protocols.
Native Chromatin Preparation and Illumina/Solexa Library Construction
) Incubate at 70°C for 30 min. ii. Purify using MinElute PCR Purification Kit or phenol:chloroform extraction. iii. Elute with or resuspend in 10 µL of 1X TE, pH 7.4. Alternatively, Klenow
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