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文献和实验Transformation Of Bacteria By Heat Shock
E.coli can be transformed using calcium, electroporation (best efficiency) or heat-shock. Competent cells for heat-shock based transformation can be easily prepared in the lab using PEG (polyethylene glycol
The biotin–avidin microplate assay is a sensitive method to measure methylation of biotinylated oligonucleotide substrates by DNA methyltransferases (MTases). The methylation reaction is carried out in solution using [methyl-3 H]-AdoMet
Using the DHFR Heat-Inducible Degron for Protein Inactivation in Schizosaccharomyces pombe
reductase, to the N-terminus of the target protein, which thereby confers temperature-sensitive degradation at the restrictive temperature. We describe here the application of this technique in the fission yeast,Schizosaccharomyces pombe .
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