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上海希言科学仪器有限公司
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文献和实验(100 μg/ mL) Adjust the pH of the resulting solution to 7.2 with the help of 1N NaOH and sterilize through 0.22 μm Millex-GV filter unit (Millipore). Store the prepared medium in small aliquots at 5° C in dark. Erythrocyte lysis buffer
Measurement of NO and NO Synthase
‐bottom or round‐bottom) Repeating pipettor (e.g., Repipettor from Eppendorf) and 5, 1.25, and 0.5 ml reservoir tips (e.g., Combitips from Eppendorf) Microtiter‐plate reader with 540‐ or 550‐nm
Marcantonio Lab Protocol Manual
when handling and be sure to dispose of properly. 2) Transfer the solution to a Beckman Quickseal ultracentrifuge tube. This can be done using a syringe and needle or by using the Beckman funnel. Top off the tube, if necessary, using CsCl/TE solution (1 g/1 ml
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