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文献和实验Purity BLOCK-iT™ siRNA resuspension volume - in vivo Purity 5 nmole 16 μl 13 μl 20 nmole 64 μl 53 μl 500 μmole
Transgenic and Knockout Mice PROTOCOL
Prior to adding the fragment-laden gel pieces, add 500µl of glassmilk. Once the gel is in the tube, melt at 55o C for 5 min (flicking the tube at least every 1 and 1/2 minutes). Then, spin out the solution for 30", wash 2X with NEW wash, spinning 30
activity 3000 Ci/mmol; Amersham Pharmacia Biotech) Drosophila Schneider SL2 tissue culture cells or other tissue or cell line for analysis, with appropriate growth facilities and equipment Ethanol (100% and 70%) Ethidium bromide (10 mg
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