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上海希言科学仪器有限公司
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文献和实验and rinse with distilled water.将预装的储液容器置于柱的顶部,以蒸馏水清洗。 4. Insert an end piece or an adaptor at the bottom of the column.将柱底部的末端或接头插入。 5. Mount the column with the packing reservoir vertically on a laboratory stand. Eliminate air from the column dead
, and the clear side of the case. Put the case in the holder, black side of the case facing black side of the holder. Run the transfer at 100V for 1 hour.3. Meanwhile, prepare 500 ml of AC Buffer (+ Tween):50 ml glycerol (= 10% glycerol)10 ml 5M NaCl (= 100mM NaCl
RLCS (Restriction Landmark cDNA Scanning) Protocol
the column for additional 1 min. Place a QIAquick spin column in a clean 1.5 mL collection tube. To elute DNA, add 50 uL TE, let stand for 1 min., and then centrifuge (13,000 rpm). Purification of target DNA using Dynabeads M-280
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