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文献和实验To the average molecular biologist working with RNA, preventing, detecting and eliminating nuclease contamination, particularly ribonuclease (RNase) contamination, is a constant and somewhat annoying challenge. The following discussion
Cloning via Restriction Digests
purification kit or QIAquick Gel Extraction kit). Prepare agarose gel. Low melting point agarose will facilitate DNA extraction at a later step, but regular agarose also works. To make a 0.8% agarose gel, use 0.8 g
. Electropherogram of Amplified aRNA Sample. Total RNA 2 µg corresponding to 60 ng mRNA) was amplified using the MessageAmp aRNA Kit (Ambion Cat# 1750) resulting in (90 µg aRNA, a 1500 fold amplification. The aRNA (900 ng) was analyzed on an Agilent 2100 bioanalyzer
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