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文献和实验Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
is observed. However, this will need to be determined on a cell type specific basis. 4) miRNA Transfection via Nucleofection and Target Selection A. Grow / expand Target ID Library cells to attain 2 x 107 cells, and trypsinize
effector cell concentration to 4 x 106 cells / ml (for commonly used E:T of 20:1), aliquot 50 µl into each well (triplicates), After 1 hour incubation, spin target cells at 1000 rpm for 5 min. Decant supernatant, resuspend
Gene Editing in One‐Cell Embryos by Zinc‐Finger and TAL Nucleases
. Meyer, M., Ortiz, O., de Angelis, M.H., Wurst, W., and Kühn, R. 2012. Modeling disease mutations by gene targeting in one‐cell mouse embryos. Proc. Natl. Acad. Sci. U.S.A. 109:9354‐9359
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