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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
off supernatant completely. 14. Resuspend the cells completely into appropriate volume of T cell isolation buffer. Use 3 mL for 250-300 million cells. 15. Proceed to magnetic separation using an LS column (Step 16).
HIGH RESOLUTION GENETIC FOOTPRINTING
TEN Buffer, 1 liter final volume (final concentration, amount of stock added to make 1 L solution) 10 mM Tris pH 8.0, 10 ml of 1 M stock 1 mM EDTA pH 8.0, 2 ml of 0.5 M stock 100 mM NaCl, 20 ml of 5 M stock water, 968 ml Lysis Buffer, 250 ml final
Myosin Light Chain Preparation
in the buffer.Spin in the J-6 for 10 minutes at 1000 rpm. Repeat for a total of 4 washes. 17.Pack the DE52 into the 5½ cm ID column in the cold room. 18.When all of the DE52 has been put in the column and has packed somewhat,take a 10 sample of the eluting
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