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文献和实验off medium from cells in T75 flask, and add serum-free medium. Replace in incubator for 30 min. Subsequently, pull off medium, wash with PBS, add 1 x trypsin/EDTA for 1 - 3 min, pull off released cells, wash flask with 20 ml of serum-free medium, pellet cells
PRINTS. These will be shown after exposing to I2 vapour. 2. Line the chamber with chromatography paper. Prepare 202 ml of solvent system (Hexane:Ether:Acetic acid 60:40:1) in a 500 ml Erlenmeyer flask. Mix and pour ~150 ml into the chamber
An Ultra-fast method of DNA extraction from Neurospora
by briefly pressing the pad between paper towels. Lyophilize for at least 2 h. 3. Grind the lyophilized pad in a 1.5 ml microfuge tube or a 15 ml conical tube using a metal rod or a spatula. 4. Add 1 ml of extraction buffer to the tube containing
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