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文献和实验SQ Blood DNA Maxi Protocol for 4-10 ml whole blood
by inverting the tube a few times. Incubate 5 minutes at room temperature. Invert the tube several times during incubation. NOTE: ERL Buffer is supplied as a 10x concentrate and must be diluted with ddH2 O according to bottle label. 2. Centrifuge
or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture
Transformation of E. coli by Electroporation
autoclaved bottles!). 4. Wash the cell pellet by resuspending in 250 ml of sterile ice-cold WB (10% redistilled glycerol, 90% distilled water, v/v). Centrifuge the cell suspension at 5,000 RPM for 15 min and carefully pour off the supernate as soon
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