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文献和实验PCR引物设计和优化(PCR PRIMER DESIGN AND REACTION OPTIMISATION)
. Thus, a sixteen base sequence will statistically be present only once in every 416 bases (=4 294 967 296, or 4 billion): this is about the size of the human or maize genome, and 1000x greater than the genome size of E. coli. Thus, the association of a greater
. Rinse with distilled water; double distilled water and ethanol. Put 1.5 mm thick spacers on the 20 x 40 cm glass plate, put the notched glass plate on top of the spacers. Clamp the plates together on one side, top and bottom with large binder
-mediated genome engineering. Cell 154(6):1370-1379. doi: 10.1016/j. cell.2013.08.022 25. DiCarlo JE, Norville JE, Mali P, Rios X, AachJ, Church GM (2013) Genome engineering inSaccharomyces cerevisiae using CRISPR-Cas systems. Nucleic Acids Res 41
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