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文献和实验CD27 分子 CD27 常用单克隆抗体或代号: VIT14,S152,OKT18A 主要表达细胞: T, Bsub [T] 分子质量(kDa)和结构: p55(TNFR- SF)(同源二聚体) 功 能: CD70的配体,T细胞活化增殖 CD27 Marker of T cell activation CD27/CD70 interactions also regulate
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
SDS lysis buffer/1x proteinase inhibitor cocktail (2.5 µl of 200 x stock from Upstate EZ ChIP kit). Roughly dissociate heads by pipetting, and transfer them into a 2 ml dounce homogenizer. Total volume is ~700 µl. �SDS lysis
A protocol for cleaning and reusing the large 25 x 25 cm plates
We regularly reuse our large 25x25 cm plating trays; initially, however, we were plagued by gross microbiological contamination when reusing the trays. Various combinations of cleaning with a laboratory dishwasher, ethyl alcohol, and UV
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