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文献和实验E-Z 96 Plant DNA Protocol-Vacuum Manifold Processing
height for elution. 10 Place the E-Z 96 DNA plate atop the vacuum manifold. 11. To elute the DNA, add 100 ul of preheated (65°C) Elution Buffer to each well using a multichannel pipet. Incubate for 5 min at room temperature. Apply
E-Z 96® M13 Isolation Vacuum Manifold Protocol
1/5 volume of MPG Buffer (200μl MPG per 1 mL culture) to the M13 supernatant and mix by vortexing. Incubate at room temperature for 15 minutes. 5. Assemble the vacuum manifold by following the manufacturer’s instructions. If using Vac-03 vacuum manifold
of panning. These single domain antibody fragments are cloned in tandem to generate manifold constructs (bivalent, biparatopic or bispecific constructs) to increase their functional affinity, to increase specificity, or to connect two independent antigen
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