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文献和实验In Situ HYBRIDIZATION TO TISSUE SECTIONS
. drop below 56 degrees C or the wax will harden rapidly. Do not let the temp. go above 62 degrees C or the wax will not polymerize correctly! Place embryos in the mold (Scientific Prod. 7275-1; peel-away disposable, 8x8mm): Cut
of gel or urea is left. Stand the gel in the lower buffer reservoir so that the notched glass plate faces the top reservoir (make sure the seal is in it’s groove) and the metal plate in the back. Clamp the gel with the metal plate
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
C 1hr 6)spin 3k rpm 5'' RT 7)add 1g/ml ground CsCl to supernatant 8)spin o/n vti50 45k rpm 9)puncture w/ 21 gauge needle 1cm from bottom, collect & pool viscous fractions 10)dyalize o/n vs. 2l TE in cold room GST FUSION
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