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文献和实验can be frozen at this point if neccessary. 10) Resuspend pellet in 150 ml of high salt buffer. 11) Rupture cells using a French press two times. --> Settings for French press: 12,000 psi, 762 gauge. 12) Spin homogenate at 5,000 rpm (7,000xg) for 15
. 11) Rupture cells using a French press two times. --> Settings for French press: 12,000 psi, 762 gauge. 12) Spin homogenate at 5,000 rpm (7,000xg) for 15 minutes using the SS-34 rotor. -->This step removes any unbroken cells and large debris
UPDATED DIRECTIONS FOR NEW BRUNSWICK MOBILE PILOT PLANT FERMENTOR
It is important to always have seal steam pressure (10-15 psi) when stirring the media, even if the unit is not in sterilization mode. This is because the steam condensate is necessary to lubricate the seal. Make sure the seal steam pressure is up to the necessary
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