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文献和实验(1000X) 0.5 ml + Glutamine (100X) 5 ml + 5% FBS (25 ml) ProcedureDraw venous blood into 60 ml syringe with 10ml 6% dextran + 7 ml citrate/citric acid. Fill to 60 ml total (blood 43 ml).Mix and sediment 30 min at RT (white blood cells now with serum
Sephadex G-50 spun column purification
ml syringe barrel with siliconized glass wool and fill to the top with a sterile suspension of Sephadex G50 equilibrated with H2 O. 2) Once filled place the syringe barrel into a 10ml centrifuge tube and spin at 3,000g for 5 minutes in a MSE Centaur
Sephadex G-50 spun column purificati
the syringe barrel into a 10ml centrifuge tube and spin at 3,000g for 5 minutes in a MSE Centaur with swingout rotor. 3) After centrifugation, 100-200ul of the required buffer is added to the top of the column and the column re-centrifuged. Repeat 3 times
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