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文献和实验.27. Column Buffers Make 500mls equivalent of base buffer: 10ml 1M Hepes pH 7.9 100ml 10% glycerol 5ml 10% NP40 (or 5.0ml 300mM Octyl glucoside) 200ml 0.5 EDTA ------- 115.2ml /5 = 23ml Base 1M KCl2.5M KCl dH20 for 0 KCL 46ml 0 0 154ml = 200
to spin without letting the free dCTP go through the column to a significant degree. Co-load some DNA to ensure that this indeed flows through under new conditions. We go about 1500rpm for about 90 seconds) 4. Dump out flow-through. Put a 1.5ml
and speed on a different machine, use isotope to see how long and how fast to spin without letting the free dCTP go through the column to a significant degree. Co-load some DNA to ensure that this indeed flows through under new conditions. We go about 1500
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