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文献和实验Pol III whole cell yeast extract (small scale)
Pol III whole cell yeast extract (small scale) Mike Schultz, Pavel Aprikian, Steve Hahn last modified6/28/99 Grow 2 liters yeast in YPD (with 3% glucose) to an A600 of 1.5 to 2.0 (inoculate ~1.5 ml saturated overnight
Replication timing by density transfer
, leu2-3,112, ura3-52, his6 in an A364A background. I aim for about 32 x 106 cells per time point. That gives enough DNA for at least 3 blots, leaving enough leeway for errors. 2. When the cell density is 2 x 106 cells/ml (OD660 ~ 0.16
Native Chromatin Preparation and Illumina/Solexa Library Construction
41.iii) LiCl buffer for ChIP Lymphocyte separation medium (LSM) Lymphocytes, human leukapheresis pack from a blood bank The pack contains leukocytes enriched by apheresis from human peripheral blood. Micrococcal nuclease
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