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文献和实验The Polymerase Chain Reaction (PCR)
. Did He Really Invent PCR? • The basic principle of replicating a piece of DNA using two primers had already been described by Gobind Khorana in 1971:– Kleppe et al. (1971) J. Mol. Biol. 56, 341-346. • Progress was limited by primer synthesis and polymerase
and a candidate gene loci are colocalized on the chromosomal region, the gene will then be cloned and sequenced. Recently, we have mapped two genetic mouse disorders, chondrodysplasia (cho ) to α1 of type XI collagen (Col11a1 ) locus on chromosome
Hints and precautions for the care, feeding and breeding of Neurospora
ascospores showing the segregation of two genes in a cross of col-4 x pyr-2. Spores were isolated on minimal agar medium in petri dishes, heated at 60° for 30 minutes for activation, and incubated at 25° for 18 hours. This nutritional mutant grows
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