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上海希言科学仪器有限公司
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文献和实验you need: Sample Buffer: 0.06M Tris-HCl, pH 6.8 10% (v/v) glycerol 2% (w/v) SDS 5% (v/v) 2-mercaptoethanol 0.0025% (w/v) bromophenol blue Grow cells overnight (~1E7 cells/ml) and collect 1.5 ml cells in 1.5 ml microfuge tube (1 minute, 14000xg).
Lipoprotein Isolation and Analysis
LDL low density lipoprotein 1.006-1.062 g/ml HDL high density lipoprotein 1.063-1.20 g/ml VHDL very high density lipoprotein >1.20
Wholemount staining of embryos
Fix embryos in formalin or MEMFA for one hour at room temperature with mixing. Rinse with TBS, replace with methanol, store at -20oC. Rehydrate by slowly adding TBS over a ten minute period. Once sample are in TBS, do 2 x
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