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文献和实验) Media LB or SOB medium for initial growth of culture LB (Luria-Bertani) Medium Per Liter: To 950 ml of deionized H2 O , add: Tryptone 10 g Yeast Extract 5 g NaCl 10 g
TISSUE PREPARATION FOR IN SITU HYBRIDIZATION
Immediately pour the solution into a one liter bottle which has been packed in ice. This cools the solution quickly and prevents breakdown of the paraformaldehyde. Store at 4°C for up to two weeks. 15% Sucrose in PBS: 500ml sterile
TISSUE PREPARATION FOR IN SITU HYBRIDIZATION
with a side-arm flask, Buchner funnel and Whatman No. 2 filter paper. Immediately pour the solution into a one liter bottle which has been packed in ice. This cools the solution quickly and prevents breakdown of the paraformaldehyde. Store at 4°C
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