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文献和实验the cell pellets in a total of 70 ml of GET/Lysozyme solution (35 ml for each bottle) by gently teasing the pellet with a spatula and incubate for 10 minutes at room temperature. (Note: Do not vortex the lysate at any time because this may shear
Large Scale Plasmid, Cosmid, BAC, PAC, and Fosmid DNA Isolation
under the same conditions. Then, the 53 ml of the culture was divided into 4X 2 liter flasks containing 1 liter of the same medium and incubated for additional 8-10 hours. After harvesting the cells by centrifugation at 5K rpm for 15 minutes in a 500 ml bottle in the RC5-B
Large Scale Plasmid, Cosmid, BAC, PAC, and Fosmid DNA Isolation by a Cleared Lys
was divided into 4X 2 liter flasks containing 1 liter of the same medium and incubated for additional 8-10 hours. After harvesting the cells by centrifugation at 5K rpm for 15 minutes in a 500 ml bottle in the RC5-B using the GS3 rotor, cell pellets
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