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文献和实验Column Purification of Demethylated Sphingomyelin
Packing column: 1) To 20 g of 100-200 mesh Bio-Sil A silica gel add 80 mls of chloroform. 2) Place a small portion of glass wool at the base of the column. 3) Pour gel solution into column and use a stir bar to press
Sephadex G-50 spun column purification
Sephadex G-50 spun column purification Spin column purification can be used to change buffers without a concomitant change in solution volume, to remove protein contaminants or to purify plasmid DNA suitable for sequencing
Column Purification of Demethylated Sphingomyelin
portion of glass wool at the base of the column. 3) Pour gel solution into column and use a stir bar to press out any bubbles in the glass wool. 4) Slowly drain the chloroform while stirring to prevent bubbles from being present in the packed gel
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