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文献和实验Expression Library Screening (Procaryotic) Using AP-Fusion Proteins
600, then dilute the cells to final OD600 of 0.5, place on ice. Screening Plaques with AP-Fusion Protein Reagents: 1M IPTG stock solution NZY plates with phage 20 x
GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
Reduced glutathione, 20 mM prepared in 50 mM Tris-HCl (pH 8.0) Isopropyl-β-D-thio-galactoside (IPTG) LB broth, containing appropriate antibiotic selection Lysis buffer: PBS + 1% Triton X-100, supplemented
mM Tris/HCl, 0.5% Tween 20, 0.5% Triton X-100 (room [4 C after addition of RNase]) B2 3 M GuHCl, 20% Tween 20 (room temp) QBT 750 mM NaCl, 50 mM MOPS, 15 % ethanol, 0.15% triton X-100, pH 7.0 (room temp.) QC 1.0 M NaCl, 50 mM MOPS, 15
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