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文献和实验Amicon Stirred Ultrafiltration Cells
. If cap assembly does not slide easily, lubricate O-ring lightly with petroleum jelly. Do not allow petroleum jelly to contact membrane! Set pressure-relief valve to horizontal (open) position. Slide cell into retaining stand, fitting ring
Amicon Stirred Ultrafiltration Cells
will rest on small ridge inside top of cell body. Introduce sample into cell. With a twisting motion, push cap down onto cell body, orienting gas inlet port on cap opposite filtrate exi
setting) and collect the mononuclear cell (MNC) (buffy coat) layer. Resuspend and wash MNCs x 3 with Dulbecco's Phosphate Buffered Saline (DPBS) with 10% Fetal Bovine Serum (FBS) x 10 min, 515 g, before plating into two 12-well plates in full EPC growth
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