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文献和实验setting) and collect the mononuclear cell (MNC) (buffy coat) layer. Resuspend and wash MNCs x 3 with Dulbecco's Phosphate Buffered Saline (DPBS) with 10% Fetal Bovine Serum (FBS) x 10 min, 515 g, before plating into two 12-well plates in full EPC growth
疟原虫 Malaria Parasites(Plasmodium)
. Department of Health, Education and Welfare, Bethesda, 1971.Fig. 1: Normal red cell; Figs. 2-18: Trophozoites (among these, Figs. 2-10 correspond to ring-stage trophozoites); Figs. 19-26: Schizonts (Fig. 26 is a ruptured schizont); Figs. 27, 28
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
above for X-gal through step 5. 6.Transfer slides to preheated PBS at 65O C and heat for 30 minutes. 7. Rinse slides in room temperature PBS for 5 minutes. 8. Rinse slides in X-Phos/NBT Detection Buffer for 10 minutes. 9. Stain slides in X
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