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文献和实验Metrics for usability of a SOLiD dataset
to some of you that I suspected that the error rate for the last 10 bases of a 35mer might be much (like 5x) higher than the rest of the read. If these quality values are to be believed, errors are more likely in the last 10 bases than the first 10 bases, but not drastically
of the specific fluor Cleavage of all bases to the 5’ of base 5 Repeat, this time querying the 9th & 10th bases After 5-7 cycles of this, perform a “reset”, in which the initial primer and all ligated portions are melted from the template and discarded. Next a new
Secondary structure of prion mRNA
++(+)a - +++ +++ +++(++)a The hairpins are present with a base-pairing probability of at least 0.8 (+++), in between 0.03 and 0.8 (++), 0.001 and 0.03 (+) and less than 0.001 (-). a A second value in parenthesis represents one species that differs from the rest of the group. b A second
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