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上海希言科学仪器有限公司
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文献和实验Clonality - X Chromosome Inactivation Assay
. Pipet upper phase into new tube and discard lower phase. Add 5 µl 3M NaAc. Add 250 µl isopropanol. Add 2 µl glycogen. Vortex briefly. Place on dry ice for 30 min. Centrifuge 14,000 rpm for 30 min. Add 300 µl 70% ethanol. Vortex briefly
and eluted from silica with 30μl 1X restriction buffer •Procedure useful for cleaning DNA from PCR reactions,restriction digests,etc.as well as purifying from agarose gel slices •If 6 M NaI solution becomes yellow,it will still bind DNA if 1/200 volume 10
DNA Extraction Protocols Using Silica
hood using PCR-free chemicals and supplies.Materials needed (suggested stocks are sufficient for several hundred "extractions"): Silica - ~100 g, Sigma # S-5631 GuSCN - ~500 g, Amresco (cheaper) # 0380 or Sigma # G-6639 Triton X-100
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