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文献和实验that should give you a good idea of what to look for if you're new to worm anatomy. Focus on the center of the sausage so that you see a nice row of nuclei on either side of the sausage. Using the fine adjuster, move the needle up/down until its very tip is in focus. Gently
Analysis of ES Cell Clones by Mini-Southern
up-and-down. Change tips between one row and the next. 9. Once the cocktail has been added to all the wells, incubate the plates overnight @ 37o C in a humidified chamber. 10. Next day, prepare the agarose gel(s) for electrophoresis. Prepare a large gel
beaker 1L graduated cylinder Stir bar Slide Box (plastic with no paper or cork liners), (e.g. #60-6306-02, PGC Scientific, Gaithersburg, MD) PCR heat cycler (e.g. DNA Engine Tetrad, MJ Research, Waltham, MA) Centrifuge with a horizontal
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