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文献和实验Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy
temperature for 4 hours and then dehydrated through a graded series of ethyl alcohols: 50% and 70% in the refrigerator (4 °C), and 85%, 95% (3 changes), and 100% (4 to 5 changes) in room temperature. The brain is left in each solution for 24 hours.
3). In the initiation step, input dsRNA is digested into 21-23 nucleotide small interfering RNAs (siRNAs), which have also been called "guide RNAs" (reviewed in 3, 18, 27). Evidence indicates that siRNAs are produced when the enzyme Dicer, a member of the RNase III
Tracking individual chromosomes with integrated arrays of Lacop sites and GFP-Laci repressor
the full chip capacity of the camera are taken of cells growing on agar or in a Ludin chamber (such that 20-30 individual cells are resolved per field). Optimal parameters for GFP are as follows: exposure time, 100-200 ms; z-spacing of 200nm for 18 focal planes
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