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文献和实验Establishment of Stable Transfectant of CHO Lec Cells
Cells should be 50~70% confluent. Detach cells with trypsin/EDTA, wash once w/ complete medium, and count cell number.Suspend cells in EPBS at ~1 x 107/ml (Typical yield is ~2 x 107 cells/flask)Add 800 µl of cells to cuvette plus DNA (dissolved
Establishment of Stable Transfectant of CHO Lec Cells
colonies in each wells necessitating you to re-clone the cells from the positive well. Try 3 x 104 cells/well (equivalent to 3 plates (300wells)/transfection) as a highest density and go down to 1 x 103 /well. If you do double transfection using
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
the last one, the incorporation of fluorescein-labeled nucleotides to DNA strands breaks, allows, using a fluorescence microscopy or a flow cytometer, to have a simple and fast method. 2. Gorczyca, W., Gong, J., Darzynkiewicz, Z. 1993. Detection of DNA strand breaks
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