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文献和实验Establishment of Stable Transfectant of CHO Lec Cells
Cells should be 50~70% confluent. Detach cells with trypsin/EDTA, wash once w/ complete medium, and count cell number.Suspend cells in EPBS at ~1 x 107/ml (Typical yield is ~2 x 107 cells/flask)Add 800 µl of cells to cuvette plus DNA (dissolved
Establishment of Stable Transfectant of CHO Lec Cells
% EtOH, mix place on dry ice, 5-10min Spin 10min at max speed Remove supernatan Wash pellet with 70% EtOH Electroporation Cells should be 50~70% confluent. Detach cells with trypsin/EDTA, wash once w/ complete medium
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
the last one, the incorporation of fluorescein-labeled nucleotides to DNA strands breaks, allows, using a fluorescence microscopy or a flow cytometer, to have a simple and fast method. 2. Gorczyca, W., Gong, J., Darzynkiewicz, Z. 1993. Detection of DNA strand breaks
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