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文献和实验Isolation of Dendritic Cells from Rat Intestinal Lymph and Spleen
Dendritic cells (DC) are rare cells in peripheral tissues, and their isolation from tissues is fraught with problems. Thus, the proportion of DC within a tissue that is extracted is unknown, isolation procedures may select for subpopulations
as displayed on the Macintosh computer data collection window. If the baseline of four-color scan lines is not flat, reclean the glass plates. 4. Attach the top buffer and the alignment brace, and fill both buffer wells with 1X MTBE electrophoresis buffer
and the alignment brace, and fill both buffer wells with 1X MTBE electrophoresis buffer. Affix the aluminum heat distribution plate by setting it on the laser stop against the glass plates. 5. Pre-electrophorese the gel for 10-30 minutes by choosing "Start Pre-run
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