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文献和实验Testing for Mycoplasma by Indirect DNA Stain (Hoechst 33258 stain)
(cell side down) onto slide. Keep slide covered with aluminum foil (Prod. No. Z18514-0 ), allowing it to set for at least 15 min at 37ºC or for 30 min at room temperature. Observe slide under uv Epi-Fluorescence at x1000. Notes
and leave for a further 3 to 5 min. Decant used fixative to toxic waste. Air dry cover-slip by resting it against the tissue culture dish for 30-120 min. Replace cover-slip in dish and add a minimum of 2ml Hoechst stain (Prod. No. H6024). Leave
Subculture of Suspension Cell Lines
to toxic waste. Air dry cover-slip by resting it against the tissue culture dish for 30-120 min. Replace cover-slip in dish and add a minimum of 2ml Hoechst stain (Prod. No. H6024 ). Leave for 5 minutes shielded from direct light by aluminum foil
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