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文献和实验How do you synthesize your dsRNA
4ul in a 1% agarose gel and use the small combs. I also us a 250bp ladder. We use the Ambion MEGAscript T7 kit (Cat.# : 1334) for the transcription reaction. Follow the Ambion MEGAscript kit protocol for Transcription Reaction Assembly and use 5 µl
combs. I also us a 250bp ladder. We use the Ambion MEGAscript T7 kit (Cat.# : 1334) for the transcription reaction. Follow the Ambion MEGAscript kit protocol for Transcription Reaction Assembly and use 5 µl of PCR template per 20 µl reaction
How do you synthesize your dsRNA
of the expected size. I run 4ul in a 1% agarose gel and use the small combs. I also us a 250bp ladder. We use the Ambion MEGAscript T7 kit (Cat.# : 1334) for the transcription reaction. Follow the Ambion MEGAscript kit protocol for Transcription Reaction
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