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文献和实验Bromodeoxyuridine Immunohistochemistry
are removed from the mother and placed quickly in a dish of ice-cold 1XPBS. 4. Fix embryo heads for 2 hr RT . Tissue samples are placed in a scintillation vial containing apx. 20ml 10 buffered formalin. For e14.5 and older embryos, the head
Quantitative Determination of Collagen Cross-links
The primary functional role of collagen is as a supporting tissue and it is now established that the aggregated forms of the collagen monomers are stabilised to provide mechanical strength by a series of intermolecular cross-links. In order
Wholemount Immuno-flurescentWholemount Immuno-flurescent
as much extraembryonic membranes as possible. Puncture the amnion and in older embryos (>E10) puncture the head with a syringe or tungsten needle. 2. Fix in 4% paraformaldehyde (PFA) in PBS at 4o C overnight. 3. Wash twice for 5-10 minutes in PBS. 4. Wash
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