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文献和实验2-D Polyacrylamide Gel Electrophoresis
chamber). 2.Start the magnetic stirrer in the mixing chamber. 3.Remove air bubbles from the tubing by opening the valve slowly. 4.Allow the tubing to fill with gel solution,then close the valve. 5.Turn on the stirrer. TIP: The stir
* 40% Acrylamide; 19:1 Acrylamide:Bis-Acrylamide -use gloves, lab coat, and dust mask when making - Acrylamide is a neurotoxin! After adding APS and TEMED, mix thoroughly and take soln' up slowly in syringe. After evacuating any air bubbles
Marcantonio Lab Protocol Manual
and plasmid DNA binds less than chromosomal DNA. 4) Insert an 18-G needle gently into the top of the tube. This acts as an air inlet to allow the plasmid DNA band to be drawn out below. 5) Recover plasmid DNA by suction with a syringe and 18-G needle
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