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文献和实验from the reservoir). 5) Sterilize the above parts by steam autoclaving at 121 °C for 60 min. Additionally autoclave one complete flow chamber, 3 x 2" segments of soft tubing, 1 male and 1 female 1/8" luer adaptor, 4 x ½" and 6 x 5/16" 8-32 flat head
Purifying Protein from Inclusion Bodies
14 until no more of the pellet dissolves. 16.Dialyze 8 M urea solutions in 4 L of a 50 mM tris-HCl buffer solution at pH 8.5 using 3.5 kD molecular weight cutoff dialysis tubing for 2 days.Repeat,dumping out buffer and replacing with fresh buffer
of interest. In addition, include a control precipitation with an (IgG) antiserum that is not directed against chromatin proteins. Cell culture from which nuclei are to be extracted (1 x 107 to 1 x 108 cells are required; see Steps 9-16) Cell culture medium
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