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文献和实验Staining protocols for placental explant cultures
. Method Fix as described in the previous protocol and rehydrate in PBS. Trim the gel using a scalpel retaining the central area with explant. Gently tease the remaining gel off the well surface using a small spatula. Use a pastette to transfer
and washes. Either leave the group in their original agar pads, or cut up the pad and place indivdual eggs in separate wells of a 9-well glass plate. Either way, trim the agarose with a clean new razor blade. 8. Rinse in NaHEPES: 2X30" 1X
Staining protocols for placental explant cultures
. Trim the gel using a scalpel retaining the central area with explant. Gently tease the remaining gel off the well surface using a small spatula. Use a pastette to transfer it to a 0.5 ml Eppendorf tube. Add blocking solution
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