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文献和实验Cocaine-stimulated Locomotor Activity Testing Protocol
3. Syringes (27 gauge, half inch hypodermic needles on disposable 1cc tuberculin syringes) are filled prior to mice being transported to the activity apparatus. Mice are injected with 20 mg/kg of cocaine in saline. The injection volume is 10 ml/kg (.1 cc
Isolation of Primary Fibroblasts from Mouse Embryos
off, transfer the embryo into the barrel of a 1cc syringe fitted with an 18G 11/2" needle and, using the syringe's plunger, force the embryo through the needle into a sterile 15 ml tube containing 1 ml of medium (DMEM, 1X GPS, and 15% FCS). 6. Working
Isolation of Primary Fibroblasts from Mouse Embryos
containing clean PBS. 5. Using a Pipetman with the end of the tip snipped off, transfer the embryo into the barrel of a 1cc syringe fitted with an 18G 11/2" needle and, using the syringe's plunger, force the embryo through the needle
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