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文献和实验Collection of Peritoneal Cells
Prepare a 10ml syringe fitted with a 26G short needle and filled with 5 to 7 ml of medium and 2 to 3 ml of air. Air is important. Prepare a Pasteur pipette with rubber pipetter. Euthanize by CO2 inhalation. Ask
Collection of Peritoneal Cells
Prepare a 10ml syringe fitted with a 26G short needle and filled with 5 to 7 ml of medium and 2 to 3 ml of air. Air is important. Prepare a Pasteur pipette with rubber pipetter. Euthanize by CO2 inhalation
for a few (about 6) filters. Heat seal bag and allow to warm in 65℃ oven for 15 minutes. Denature the radiolabelled DNA probe (1-2 x 106 cpm/filter) at 95℃, 5-10 minutes. Add probe at corner of sealed bag using a 25G needle and syringe. Heat seal below the injection site
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