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上海希言科学仪器有限公司
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文献和实验(cells/ml) = B x C x D Total number of viable cells = concentration of viable cells x volume Total number of cells = number of viable + number of dead cells Percentage Viability = (No of viable cells x 100) divided by Total No of cells Key Points
Bromodeoxyuridine Immunohistochemistry
between the following steps, and use apx. 20 ml for each reagent (step 3-7). 6. 30, 50, 70, 95, 100 x 2, RT, 30 min. each , with slight agitation. This step functions to dehydrate the tissues through an increasing ethanol series: Samples in 70, 95, or 100
Subculture of Suspension Cell Lines
2 x Thioglycollate Medium (TGM) (Prod.No. F4797 ) and 2 x Tryptone Soya broth (TSB) (Prod.No. T8907 ) with 1.5ml test sample. Inoculate 2 (TGM) and 2 (TSB) with 0.1ml C.albicans (containing 100 colony forming units, cfu). Inoculate 2 (TGM
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