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文献和实验Inverse PCR & Cycle Sequencing
, dry. 11) Resuspend in 150 µl TE. 12) Store at -20°. Buffer A 100 mM Tris-HCl, pH 7.5 100 mM EDTA 100 mM NaCl 0.5% SDS LiCl/KAc Solution Mix 1 part 5 M KAc stock : 2.5 parts 6 M LiCl stock II. Digestions
Preparation of Nested Deletions
filters to cool, then individually wet in 2 x SSC (in a 150 mm petri dish; need about 50 ml), followed by immersion in prewarmed prehybridization solution contained also in a 150 mm petri dish supported in a plastic box. Put top on box
cDNA Library Screening Protocol - ZAPII
10) Allow filters to cool, then individually wet in 2 x SSC (in a 150 mm petri dish; need about 50 ml), followed by immersion in prewarmed prehybridization solution contained also in a 150 mm petri dish supported in a plastic box. Put top on box
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