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文献和实验Hints and precautions for the care, feeding and breeding of Neurospora
their contents (P. St. Lawrence), and are conveniently stored for future use. 8. Heatshock. For heatshock, a 60°C water bath is preferable to a hot-air oven. (Greater latent heat, better heat transfer, more stable). Allow at least 30 minutes at 60°C to assure
Hints and precautions for the care, feeding and breeding of Neurospora
water bath is preferable to a hot-air oven. (Greater latent heat, better heat transfer, more stable). Allow at least 30 minutes at 60°C to assure killing of vegetative cells with a water bath, double this for dry air. Cover the bath to assure killing
to polymerize about 1 hour (this depends on the temperature of the acrylamide, as well as how much temed and APS is added; again I suggest warming the acrylamide up to allow for rapid polymerization). If not running the gel immediately, cover with saran
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