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文献和实验Protocol for vacuum-infiltration transformation of Arabidopsis thaliana
., ecotype Wassilevskija (WS) was used for all the experiments and to generate a collection of T-DNA insertional mutants. The C58C1 (pMP90) strain of Agrobacterium tumefaciens, containing the pTiC58- based helper plasmid pMP90 and the binary vector pGKB
PIC Cross-linking and Immune Precipitation
and form 2X PICs (200 microliter)per standard protocol5.Wash PICs 3 x 400 microliter txn wash buffer6.Resuspend PICs in 200 microliter txn wash buffer7.Do not split reactions into 100 microliter aliquots as is standard for cross-linking reactions8.Cross
: 400 mL of PBS x1 + 10 mL of PBS-MgCl2 (MgCm2=4g/L) + 10 mL PBS-CaCl2 (CaCl2= 5.3 g/L) Working Solution of Thrombin = 7 µl of Thrombin (1250U/mL) + 1 mL of Clot Retraction Buffer ADDITIONAL INFO The Clot Retraction
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