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文献和实验HIGH RESOLUTION GENETIC FOOTPRINTING
-stranded DNA binding resin (Clontech), followed by Quiaquick PCR purification kit (Quiagen). The purified PCR product is then digested with the restriction enzyme whose recognition site is unique to the replacement oligonucleotide, to generate a ladder
0.5% 0.5% 0.5% 0.5% 0.5% 0.5% 0.5% 0.5% 0.5% - 0.5% 0.5% 0.5% 0.5% FBS - - - - - - - - - 10% - - - - KnockOut Serum Replacement 20% - - - - - - - - - - - - - L-glutamine 2 mM 2 mM 2 mM 2 mM 2 mM 2 mM 2 mM 2 mM 2 mM
cutaneous T cell lymphomas, AJCP 2001;116:72 Negative staining: normal B cells, most cutaneous peripheral B cell lymphomas (? reflects replacement of reactive pattern containing dendritic cells with a neoplastic pattern of B cells) Micro images:
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