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文献和实验Protein Turnover Methods in Single-Celled Organisms: Dynamic SILAC
of metabolic label is dictated by several factors, including abundance in the proteome, metabolic redistribution of the label in the precursor pool, and the downstream mass spectrometric analytical protocols. Key issues include the need to control
, the whitefish blastula has long served as a model of mitotic division in animals. It also has the advantage of demonstrating clear spindle formation in the cytoplasm. 4.Using Figure 11.3A as a guide, draw and label all stages of mitosis, including spindle
Label-free strategies for quantitative proteomics provide a versatile and economical alternative to labeling-based proteomics strategies. We have shown for different types of biological samples that spectral counting-based label-free
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