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文献和实验丁香园myl0605的观点:前几天为自己养的PC12是未分化还是分化型困扰,查了一些资料,现发上来希望能给有同样问题的战友有一点帮助!培养条件:PC12细胞培养于铺有鼠尾胶原的玻璃培养瓶中,置于CO2培养箱(37℃,95%空气5%CO2),培养基选用DMEM(pH7.4,高糖),加入10%灭活小牛血清及5%灭活马血清。分化前:PC12细胞在培养基中多呈圆形,直径15~20μm,也有椭圆或多角形的,扁平的细胞少见,倾向于聚集成小团簇状(A图)。分化后:加入NGF后的24h内细胞停止分裂,长出似
Evaluation of PCB Dechlorination in Sediments
Polychlorinated biphenyls (PCBs) are important environmental contaml- nants. They are widely distributed and have been associated with toxic effects in wildlife, such as deformities and lowered reproductive success (1 ), and with hepatic
Methods for Evaluation of PCB Dechlorination in Sediments
chlorinated congeners formed tend to be more aerobically degradable and less toxic. Remedial action plans for PCB-contaminated sediments should therefore take mto account the extent of dechlorination that has occurred and the potential
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